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E S6) in order to determine the spacing between the two target sites that would result in the highest activity of the Bat-FokI fusion proteins. As a negative control, we tested a template with a control sequence instead of the Bat1 target sites. Bat1-FokI and dTALEBat1mimic -FokI were expressed in vitro and equal volumes of reaction product were incubated with the target DNA. After 3 h at 37 C the
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Ingle interventions (0.38 ?0.02 andABB*Figure 1 Systolic blood pressure in pregnant and non-pregnant guinea-pigs treated with angiotensin II (AII), bradyzide (BDZ), or AII + BDZ. Systolic blood pressure on gestational days 34 (A) and 60 (B) in controls (C), AII, BDZ and AII + BDZ treated dams and in AII + BDZ-treated non-pregnant females (AII + BDZ NP). The broken line depicts the mean systolic bl
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Ange values.Purification of Tric1SAMThe gene fragment corresponding to the SAM domain of At3g49560/Tric1 (amino acids 191?61) was cloned in the pET15b vector (Novagen), yielding an N-terminal His tag fusion protein. Mutations corresponding to the Tric1SAM variants K15A and K20A were introduced by site-directed mutagenesis using the Quikchange II-XL kit (Agilent Technologies). The expression vector
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E S6) in order to determine the spacing between the two target sites that would result in the highest activity of the Bat-FokI fusion proteins. As a negative control, we tested a template with a control sequence instead of the Bat1 target sites. Bat1-FokI and dTALEBat1mimic -FokI were expressed in vitro and equal volumes of reaction product were incubated with the target DNA. After 3 h at 37 C the
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Karyotic cell nucleus and endomembrane system as per the chimeric model. The key event in the origin of the eukaryotic cell is postulated to be a symbiotic association between a gram-negative eubacterium (from the proteobacteria-1 group) and likely an "eocyte" archaebacterium. This association led to the loss of the outer membrane from the gram-negative bacterium (not shown). As the membrane of th
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Englishlanguage randomized trials for acupuncture and about half of those concerned discomfort.The clear and obvious goal of such trials is usually to Vickers et al; licensee BioMed Central Ltd.This really is an Open Access write-up distributed beneath the terms in the Creative Commons Attribution License (creat.Englishlanguage randomized trials for acupuncture and around half of those concerned p
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Myometrial spiral arteries, the perimeter occupied by endoluminal EVT was reduced in the groups receiving AII, BDZ, (P
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