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Not yet known what causes these rapid changes. They may result from the presence of bacteria, the influx of immune cells to combat the bacteria, or the release of mediator(s) from immune cells or epithelium to deal with the insult. We speculate that the rapidity of the response (within 4 hr) is due to the release of stored mediators, such as chemokines, rather than due to the synthesis and secreti
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Ation of stathmin, indicating that LPS activation of Cdk1 kinase is the major contributing factor for rapid stathmin destruction. Our studies focused on early time points of macrophage classical activation when the major morphological and phenotypic events are occurring. We demonstrate that the classically activated macrophages adopt distinct cell morphologies and that stathmin reduction is requir
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Or dye at 37 , the fluorescence was measured at an excitation wavelength of 530 ?25 nm and an emission wavelength of 590 ?35 nm with a Bio-Tek FL600 microplate fluorescence reader. Triplicate samples were assayed, background corrected and the results were expressed as a percentage of the values from untreated control cells. Cytokine protein arrays Cytokine expression profiling was performed on cul
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Ving pathogens , these types of vaccines are usually safe compared to
Ving pathogens , these types of vaccines are usually safe compared to live attenuated vaccines. Overall, these technologies have allowed to achieve the successes of vaccinology in the last century and to produce the vaccine formulations available on the market. However many new vaccines are needed and for them , [8] new str
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To the cell surface (Fig. 7). Continued signaling through TLR4 is important for NF- B-mediated gene expression and secretion of pro-inflammatory cytokines in macrophages during a pathogenic challenge (9). Here we show that stathmin can modulate this sustained signaling, likely by affecting the stability of MT to enhance intracellular trafficking of receptor-containing vesicles, MMPs, and cytokines
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MRNA expression of CD163 (left panal) and M1 polarization was evaluated by HLA-DRA mRNA expression (right panal). b Explant mammary gland sections were subjected to immunohistochemical analysis, stained for CD163 (left panel) or HLA-DRA (right panel) and images were captured at 100X. Representative pictures are displayed for tissues from each treatment group which was performed in triplicate sampl
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Ors approved the final version of the manuscript. Acknowledgments--We thank Sofia Pustylnik for generating the stathmin-GFP construct, Carlo Dumpit for assistance with SEM analysis, Urja Naik for assistance with C3bi-sRBC binding assays, and Umama Siddiqi for helping with NF- B translocation analysis. We also thank Dr. He Song Sun for critical reading of the manuscript.
THE JOURNAL OF BIOLOGI
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An Online Marketing Service must have the ability to take your business to an incredible new level and make you stand out uniquely to all the users and viewers of the internet. This type of promotional company must furthermore market a business to intense levels so that clients are able to find you easily and quickly. There must be absolutely no doubt that the company is capable of offering qualit
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In Figure 1. Case reading and reporting was mostly done by cardiologists (70 ), or a team of cardiologists and radiologists (26 ). Image quality was found to be good or excellent in 91 , moderate in 8 , and inadequate in 1 of cases. Severe complications occurred in a minority of patients (0.07 ) and were all associated with stress testing. We reported NSVT (n = 1), VF (n = 1) during dobutamine in
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