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A promoter. If the NH2-terminus is sufficient to confer Cullin binding specificity, all chimeras with the same NH2-terminal Roc sequence should bind the same Cullins, regardless of the RING domain to which they are fused. We transfected these constructs into S2 cells and determined Cullin binding by IP/immunoblot. Because we had already observed that Roc1a binds Cul1 and Cul3 but not Cul5, thatRoc
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